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Fig. 6 Mitochondrial association with autophagosome membranes is not disrupted by VapB or VapBP56S expression. ??C, Deconvolved images of pLL axon terminals coexpressing mitochondrially localized GFP (green) and RFP-LC3 to label autophagosomes (RFP in magenta in merge, black on the right inset) in WT (A), VapB transgenic (B), or VapBP56S transgenic (C). Inset below; yellow circles denote mitochondria-associated autophagosome; blue circles denote unassociated autophagosomes. D, Proportion of the mitochondrial volume colocalized with autophagosomes normalized to mitochondrial volume. E, Proportion of the mitochondrial volume colocalized with autophagosomes normalized to autophagosome volume. F, Average autophagosome volume in the axon terminal. G?I, Example mitochondria from deconvolved images of axon terminals of WT (G), VapB transgenic (H), or VapBP56S transgenic (I). J, Average mitochondrial volume in the axon terminal. Scale bar: 5 ?m in A; 1 ?m in A inset and G. Each data point for D?F represents the average calculated from an individual animal. Data points in J represent 158 mitochondria from WT, 10; VapB, 8; P56S, 8 animals per group. All data represented as mean ± SEM. ANOVA with Dunnett's post hoc contrasts in D, E, and F. Kruskal?Wallis with Dunn's post hoc contrasts in J.