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Fig. 2 6-OHDA selectively reduces th1 expression and induces a state of mitochondrial dysfunction through ROS introduction. RT-qPCR of four groups exposed to 30 ?M 6-OHDA for 24 h starting at day 3 (Strain AB, 24 embryos pooled per point). A significant reduction compared to controls occurred for th1, a marker for dopaminergic cells, and pink1, a marker for mitochondrial dysfunction (A). A significant increase compared to controls for sod1 and sod2, part of the cells ROS response, were observed (B). Three genes did not show a significant reduction: gad1b, a marker for GABAergic neurons; slc6a3, the DA transporter; and slc18a2, a vesicular monoamine transporter (C) (normalized to hprt1 and control, *Indicates a p < 0.05). ROS, reactive oxygen species; RT-qPCR, reverse transcriptase-quantitative polymerase chain reaction; DA, dopamine.