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Fig. 5 Establishment of distinct molecular signature for mid-suture mesenchyme A Dot plot shows shared and cell type-specific markers for subsets of mesenchyme. B Violin plot split by age for genes expressed in regulatory mesenchyme. C In situ analysis of juvenile frontal bone and adult coronal suture. Dotted lines outline the frontal (F) and parietal (P) bones. Arrows mark bone front and suture mesenchyme, arrowheads the periosteum. D, E Double in situ analysis of adult coronal suture. Orange boxed insets show expression of fli1a and hyal4, but not grem1a, at suture edges, and red boxed insets show grem1a but not fli1a expression in mid-suture region. In situs were performed in biological triplicate. Scalebars: 100 μM.