ZFIN Image: Farmer et al., 2024, Fig. 4

Image

Figure Caption

Fig. 4 Spatially restricted osteogenesis at bone fronts and suture edges. A Confocal image of juvenile skull (dorsal view) showing osteoblast:nlsEOS relative to the osteoblast marker sp7:mCherry in the frontal (F) and parietal (P) bones. N = 6. B Schematic of treatment scheme for juvenile calvaria. Red box indicates area imaged in the next panels. C, D Confocal images of osteoblast:nlsEOS+ frontal bone 24 h post-conversion (hpc) showing merged and individual channels, and extracted de novo osteoblasts (green only). N = 3. E Schematic of treatment scheme for adult calvaria. Red box indicates area imaged in the next panels. F, G Confocal images of osteoblast:nlsEOS+ sagittal suture 7 days post-conversion (dpc) showing merged and individual channels, and extracted de novo osteoblasts (green only). Scalebars: 100 μM (A), 50 μM (C, F).

Acknowledgments

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