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Fig. 1 Single-cell RNA-sequencing analysis of juvenile and adult calvaria. A Schematic of dissected calvaria from juvenile and adult zebrafish. B Uniform Manifold Approximation and Projection (UMAP) plot of integrated juvenile (16,245 cells) and adult (5066 cells) datasets. Connective and skeletogenic clusters outlined by dashed lines. C UMAP analysis of reclustered connective and skeletogenic subset, including juvenile (4324 cells) and adult (4595 cells) datasets. Cell identities grouped by circles. D UMAP plot of osteoblast and prrx1a+ mesenchyme subset (517 juvenile cells, 656 adult cells). E Dot plot showing markers enriched for each cluster within the osteogenic/mesenchymal subset. F RNA velocity (arrows) showing differentiation flow for cells within the osteogenic/mesenchymal subset. G, H Dot plots showing zebrafish paralogs for markers of osteogenic and meningeal clusters in the mouse coronal or frontal suture datasets mapped onto the zebrafish osteoblast/mesenchyme subset.