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Fig. 8 Western blot analysis of effects of compound 5x in MCF-7 cell line on Hsp90 client protein levels (ER?, IGF1R, MEK, ERK 1/2) and representative heat shock proteins (Hsp70, Hsp90) after 24 hours of treatment. A) Representative Western blot images; B) Quantification results normalised on GAPDH levels. The bars represent mean values with SD and Welch?s t-test was used to evaluate statistical significancy (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). Full images used for quantification are shown in Supplementary information Figures 11S-14S .
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