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Fig. 4 Determination of reactive oxygen species (ROS) in unstimulated control (control - cells incubated with media only), control 2 (cells incubated with ROS-sensitive fluorescent indicator (H2DCFDA), pure brusatol (38.66 nM), pure docetaxel (60 nM), mixture of pure drugs (38.66 nM brusatol + 60 nM docetaxel), and nanoparticle formulations containing equivalents of the pure drug mixture at 2 h, and 24 h in LNCaP cells (left) and PC-3 cells (right).
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