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Fig. 2 gphnbvo8/vo84 mutants exhibit normal gross morphology and xanthine oxidase activity. A Representative images of WT and gphnbvo84/vo84 zebrafish larvae at 5 dpf. B The body length of larvae at 5 dpf. nWT = 13 animals, ngphnb = 12 animals, 2 technical replicates. t[0.7040], df[23]. C Fish survival tracked from larvae to adults. 3 groups of 50 fish were tested per condition. Fwpf[2.289, 9.156] = 144.2, Fgenotype[1, 4] = 20.58, Finteraction[15, 60] = 16.14, Ftank[4, 60] = 67.54. D Representative images of 1.5 years old adult zebrafish of the indicated genotypes and sexes. E Xanthine oxidase activity measured by fluorometric assays over time in WT and gphnbvo84/vo84. Each trace represents the total activity of 15 animals combined at 5 dpf. F Quantification of the xanthine oxidase activity at the end of recording from (E). Two sets of homogenates, each consisting of 15 animals were tested for each condition. t[0.7709], df[2]. All data are represented as mean ± SEM; ns, not significant; *, p < 0.05; (B,F) unpaired t-tests; (C) two-way repeat measure ANOVA with Sidak’s multiple comparison test