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Fig. 3 timp2b expression is upregulated in hearts contracting at a reduced rate. (A-C) Relative mRNA levels of timp2b, timp2a, mmp2, mmp14a and mmp14b from extracted hearts of tnnt2a morphants and uninjected controls at 50 (A), 74 (B) and 98 hpf (C). (D) Relative mRNA levels of timp2b, timp2a, mmp2, mmp14a and mmp14b from extracted hearts of 74 hpf zebrafish treated for 24 h with BDM or DMSO. (E) Relative timp2b mRNA levels from extracted hearts of 74 hpf zebrafish treated for 24 h with Tricaine or DMSO. (F) Relative timp2b mRNA levels from extracted hearts of 50, 74 and 98 hpf zebrafish treated for 24 h with IBMX or DMSO. timp2b expression is significantly upregulated upon reduced cardiac contraction (tnnt2a morpholino, BDM and Tricaine treatments), but remains unaffected upon increased heart rate (IBMX treatment). n=4 biological replicates, 25 hearts each. Plot values represent mean±s.d.; P-values determined by Mann–Whitney U test. FC, fold change. See Table S6 for Ct values.