|
Fig. 2 Mechanical forces regulate cECM dynamics in a region-specific manner. (A-F′) Wild-type hearts before (50 hpf; A,A′,C,C′,E,E′) and after (74 hpf; B,B′,D,D′,F,F′) 24-h treatments with DMSO control (A-B′), IBMX (C-D′) or BDM (E-F′). Single-plane images are shown in A-F and 3D surface renderings in A′-F′. Images before and after treatment were taken of the same animals. (G-I) cECM index of IBMX- or BDM-treated hearts compared with DMSO-treated hearts at 74 hpf. A reduction of the ventricular ECM index was observed in IBMX- and BDM-treated hearts (G). For the AVC (H) and atrial (I) ECM indices, increases were observed in BDM-treated hearts, whereas no changes were observed in IBMX-treated hearts (H,I). n=7 for DMSO, 8 for IBMX, and 7 for BDM (G-I); plot values represent mean±s.d.; P-values determined by one-way ANOVA followed by multiple comparisons with Dunn test. Scale bars: 30 µm. A, atrium; AVC, atrioventricular canal; V, ventricle.