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Fig. 4 Window-specific AA exposure resulted in differential morphological responses. (A) Heatmaps showing the mean incidence (n = 3; each replicate has 20 embryos) of malformation (120 hpf) and mortality (120 hpf) after embryos exposed to 0, 2, 4, 8, and 16 μM AA from 8–120 hpf (I), 8–24 hpf (II), 24–48 hpf (III), and 48–72 hpf (IV). (B) Representative images showing larval eye size (area within dotted circles) at 120 hpf after exposed to 0, 4, and 16 μM AA from 8–24 hpf and 48–72 hpf. (C) The ratio of larval eye size (area) to body length at 120 hpf after exposed to 0, 2, 4, 8, and 16 μM AA from 8–24 hpf (n = 14–16) and 48–72 hpf (n = 12). Values are plotted as mean + SD. *P < 0.05 and **P < 0.001 indicate significant differences from the vehicle control.