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Fig. 3 Continuous AA exposure (8–120 hpf) affected axonal growth, induced oxidative stress, and cell apoptosis, but had no effect on muscle fiber. (A) A schematic diagram showing zebrafish embryos exposed to 0, 1, and 2 μM AA from 8–120 hpf and sampled at various time for different endpoints. (B) Representative images showing axonal growth (white arrows) in the primary motor neuron in Tg (NBT: MAPT-GFP) fish at 48 hpf. Asterisks indicate axons with extra proliferated branches. (C) Representative histological sections of muscle fibers (black arrows) from wild type AB line fish at 120 hpf. (D) Oxidative stress measured by MDA, SOD and ATP and cell apoptosis measured by Caspase 3 in wild type AB line fish at 120 hpf. Values are plotted as mean + SD (n = 3; each replicate consists of tissue samples pooled from 20 larvae). *P < 0.05, **P < 0.001 indicate significant differences from the vehicle control.