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Fig. 2 mTorC1 activation in tsc2vu242 fish habenulas (A) Representative optical sections through habenula of tsc2vu242/vu242 fish and their wild-type tsc2+/+ siblings at 5, 10, 15, and 20 μm from the top. pRps6—yellow, nuclei—blue. L—left habenula, R—right habenula, P—pineal complex. Scale bars, 20 μm. (B) Number of pRps6-positive cells in LdHb of tsc2vu242 fish (5 μm: tsc2vu242/vu242 control vs. tsc2+/+ control p = 0.004 and vs. tsc2vu242/vu242 treated with RAPA p = 1.038 × 10−5; 10 μm: tsc2vu242/vu242 control vs. tsc2+/+ control p = 4.656 × 10−5 and vs. tsc2vu242/vu242 treated with RAPA p = 1.038 × 10−5; 15 μm: tsc2vu242/vu242 control vs. tsc2+/+ control p = 1.584 × 10−5 and vs. tsc2vu242/vu242 treated with RAPA p = 1.038 × 10−5; 20 μm: tsc2vu242/vu242 control vs. tsc2+/+ control p = 0.004 and vs. tsc2vu242/vu242 treated with RAPA p = 1.038 × 10−5). The dots on the boxplots represent the number of fish in the experiment (N > 10 per experimental group). (C) Quantification of mean intensity of pRps6 fluorescence from LdHb of tsc2vu242 fish (5 μm: tsc2vu242/vu242 control vs. tsc2+/+ control p = 0.0051 and vs. tsc2vu242/vu242 treated with RAPA p = 1.0912 × 10−5; 10 μm: tsc2vu242/vu242 control vs. tsc2+/+ control p = 0.0017 and vs. tsc2vu242/vu242 treated with RAPA p = 1.0912 × 10−5; 15 μm: tsc2vu242/vu242 control vs. tsc2+/+ control p = 0.001 and vs. tsc2vu242/vu242 treated with RAPA p = 1.0912 × 10−5; 20 μm: tsc2vu242/vu242 control vs. tsc2+/+ control p = 0.0007 and vs. tsc2vu242/vu242 treated with RAPA p = 1.0912 × 10−5). The dots on the boxplots represent the number of fish in the experiment (N > 10 per experimental group).