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Fig. 2 CRISPR/Cas9 dre-ca8 F0 knockout does not trigger obvious gross developmental defects. (A) sgRNA genomic target locations on dre-ca8 sequence. (B,C) ca8 CRISPR/Cas9 F0 knockout did not trigger any obvious toxicity. (D) Although of normal appearance, crispant animals present a slightly reduced body length at 3 dpf that becomes non-significant from 7 dpf. dpf, days post fertilization. [Color figure can be viewed at wileyonlinelibrary.com]
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