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Fig. 3 Macrophages interacts with, and uptakes, apoptosing cells. (A) A representative image of a mpeg1:GFP host embryo with wild-type donor cells labeled in cascade blue. Individual fluorescent channels are shown for cascade blue donor cells (B) and GFP labeled macrophages (C). (D) A mpeg1:GFP host embryo containing transplanted wild-type cells that lack the actc1b:epNTR transgene shows macrophages (green) that do not linger within muscle tissue. (E) A mpeg1:GFP host embryo with transplanted actc1b:epNTR muscle cells (magenta) treated with MTZ and imaged 1 day after washout. Embryos show increased residence of macrophage cells in ablated area. (F) A mpeg1:GFP macrophage in an actc1b:epNTR embryo. mCherry puncta indicates uptake of ablated muscle debris from injured cells (white arrows). Scale Bars = 50 ?M