Fig. 8 Mean intensity score of fixed AR-stained bones of 5 dpf larvae treated with proprietary compound 9. A) shows the results of replicate 1 and B) shows the results of replicate 2. All groups were compared to the solvent control group. Significant differences between the solvent control and any of the other groups are indicated. P ≤ 0.05 (*), p ≤ 0.01 (**) and p ≤ 0.001 (***). The color of the asterisks indicate that the solvent control was significantly more (black) or less (green) intense stained than the other group. The green underlined asterisks indicate that the solvent control was significantly less stained than the medium control.
Reprinted from Reproductive toxicology (Elmsford, N.Y.), 127, Hoyberghs, J., Ball, J., Trznadel, M., Beekhuijzen, M., Burbank, M., Wilhelmi, P., Muriana, A., Powles-Glover, N., Letamendia, A., Van Cruchten, S., Biological variability hampers the use of skeletal staining methods in zebrafish embryo developmental toxicity assays, 108615, Copyright (2024) with permission from Elsevier. Full text @ Reprod. Toxicol.