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Fig. 4 Total distance moved (mean ± 95 % confidence interval) by 4.5 d old zebrafish (Danio rerio) embryos in the green light-dark locomotion test, in response to exposure of parents and/or embryos to PTU during the a) dark phases (10 × 10 min.) or b) green light phases (10 × 10 min.). Replicates and individual observations: control: 5 replicates, N = 109; embryonic exposure: 6 replicates, N = 131; parental exposure: 4 replicates, N = 88; combined parental and embryonic exposure: 5 replicates, N = 104. Different letters: significant differences between groups by Post-hoc Tukey test; capital and lowercase letters: differences in the main effect of embryonic exposure. * Statistically significant effect of main effect parental exposure (p < 0.05) by LMM and Kenward-Roger F-statistics (see Table 2).