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Fig. 1 Exposure design of iFEDT experiment and cross design experiment: Zebrafish (Danio rerio) parents and offspring were exposed to PTU (iFEDT, results published in Pannetier et al. 2023a and Goelz et al. 2023). For the present study, eggs from the non-exposed parental generation (0 mg PTU/L) were exposed to either 0 or 78 mg PTU /L. Likewise, eggs from the parental generation exposed to 78 mg PTU/L were exposed to either 0 or 78 mg PTU /L. Between 4 and 18 days of exposure of the parental generation, eggs were collected and directly exposed for 4.5 d for evaluation of photomotor response and gene expression, or for 5 d for measurement of area of swim bladder.