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Fig. 8 Wnt signaling mutants have lower expression of metabolism proteins. Mass spectrometry was performed on embryos at 30% epiboly. (A) Only samples with similar total ionic chromatograms were used, resulting in only one biological replicate for each genotype. (B) Heat map of 53 differentially expressed proteins using PEAKS 10 software (FDR < 0.1, logFC ≥ 1, peptides ≥ 2). (C) Eighteen proteins were identified to have a connection to Wnt signaling in the literature: Fabp3 (Liu et al., 2013), Aldh2.2 (Zhao et al., 2015), Prdx1 (Zheng et al., 2018), Idhba (Mazzio et al., 2020), Pcxa (Lee et al., 2012), Tcp1 (Tang et al., 2020), Igf2bp3 (Song et al., 2023), Pkma (Lu et al., 2021), Actb2 (Gu et al., 2021), Cct5 (Li et al., 2022b), Prdx6 (Xu et al., 2019), Tpi1a (Xia et al., 2023), Prdx2 (Feng et al., 2020), Ppa1b (Niu et al., 2017), Sod1 (Chandrasekharan et al., 2021), Cox17 (Li et al., 2022a), Ranbp1 (Liu et al., 2019), Stmn1a (Zhang et al., 2019). (D and E) String protein networks were created using all proteins identified (D) and a network of the 39 nonribosomal proteins (E).