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Fig. 1 Wnt regulator mutants have developmental defects. (A) 2 dpf Zebrafish embryos were observed for heart looping phenotypes and quantified in (B). (C) Zebrafish were treated with PTU from 1–5 dpf and then fixed at 5 dpf for neuromast staining with terminal neuromasts highlighted by the red box and quantified in (D). (E) Maternal-zygotic adult axin2gh1/gh1 zebrafish occasionally develop a curved spine phenotype. (F) Adults from a heterozygous incross were phenotyped for the curved spine followed by genotyping. (G) Maternal-zygotic adult axin2gh1/gh1 zebrafish often have a receding operculum phenotype. (H) Adults from a heterozygous incross were phenotyped for the receding operculum followed by genotyping. Error bars represent SEM, and a Chi-Square analysis was used with **** = p value < 0.0001.