Fig. 1

Fig. 1

Zebrafish cacna1da mutant allele (sa17298) and its molecular consequence. a Schematic representation of the zebrafish cacna1da transcript. Red bars represent exons and lines represent introns. Image retrieved from ensembl.org. b Schematic representation of zebrafish cacna1da and sa17298 mutation. Nucleotides in upper case are within the exon while those in lower case are within the intron. c Sample gel electrophoresis of BstEII restriction digest and resulting PCR products to determine genotype of fish/larvae. BstEII does not cut homozygous sa17298, but cuts WT into two bands (70 and 119 bp) and heterozygous sa17298 into three bands (70, 119 and 196 bp). Note the multiple bands in the BstEII digested versus the undigested corresponding samples. L: 1 kilobase DNA ladder, +/+: cacna1da^WT/WT, +/−: cacna1da^sa17298/WT, −/−: cacna1da^{sa17298/sa17298}.dcacna1da mRNA levels as measured by agarose gel electrophoresis (rt-PCR). ecacna1da mRNA levels as measured by qPCR. cacna1da mRNA expression is normalised against gapdh and rps18