Fig. 1.

Fig. 1.

Overfed zMIR/VDBP zebrafish exhibit obesity, hyperglycemia and glomerular filtration barrier dysfunction. (A) Experiment schema of overfeeding to induce diabetes. Female VDBP and zMIR/VDBP zebrafish were divided into normal-feeding (NF) and overfeeding (OF) groups. Proteinuria measurements were performed every 2 weeks for a total of 20 weeks. At the endpoint, renal histopathology was analyzed using Hematoxylin and Eosin staining and transmission electron microscopy. (B) Representative images of VDBP and zMIR/VDBP zebrafish with NF and OF for 20 weeks. (C,D) Quantification of body weight (BW) (C) and fasting blood glucose (FBG) (D) of the four groups at the endpoint of the feeding experiment (n=5). (E) Proteinuria changes in the urine every 2 weeks during the overfeeding experiment in overfed VDBP and zMIR/VDBP zebrafish (n=5-22). The VDBP-GFP signal was measured using ELISA as an indicator of albuminuria. Data are presented as means±s.e.m. ns, not significant; ***P<0.001 versus VDBP with OF; Bonferroni–Dunn multiple comparisons test.