ZFIN Image: Gronseth et al., 2024, Figure 4

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Figure Caption

Figure 4

Synaptic vesicle release regulates oligodendrocyte-axon interactions in a neuron subtype specific manner. (A) Lateral view confocal images of GFP labeled axon interacting with oligodendrocyte processes labeled with RFP (anterior left, dorsal side up). White arrowheads point to sites of GFP-RFP colocalization. Scale bar = 5 μm, 1 μm (inset). (B) Graphs show the interaction index, which is defined as the percentage length of axon segments covered by interacting processes. Individual points represent the percent coverage of an individual axon. Sample size = number of animals, number of axons. Reticulospinal = 20, 20 (control), 19, 20 (TeNT-LC); CoPA = 18, 18 (control), 6, 8 (TeNT-LC); 5-HT = 19, 35 (control), 11, 24 (TeNT-LC); DDT = 23, 32 (control), 13, 24 (TeNT-LC). Black bars (controls) and blue bars (TeNT-LC) represent mean ± SEM. The p-values were derived using either a parametric t-test (Reticulospinals, CoPA) or Mann–Whitney test (5-HT, DDT) based on whether data showed a normal distribution.

Acknowledgments

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