Figure 4—figure supplement 1—source data 3.

Figure 4—figure supplement 1—source data 3. Identification of Styxl2-interacting partners by BioID. (A) A representative gel of samples from BioID before mass spectrometry analysis. Whole cell lysates from control (Ctrl) and Doxycycline-treated (Dox) cells were subjected to Western blot using Streptavidin-HRP (top panel) or an anti-Styxl2 antibody (middle panel). (B) The biotinylated proteins identified by mass spectrometry were plotted with the log2Enrichment scores on the X-axis and the minus log10(p.value) on the Y-axis. The Enrichment score denotes the ratio of the peak intensity of reporter ions. Red dots indicate proteins with the Enrichment score >1.2 and p-value <0.05. Blue dots indicate proteins with the Enrichment score <0.83 and p-value <0.05. (C) Selected candidate proteins with more than 10 recovered peptides were shown.