Figure 2—figure supplement 1.

Figure 2—figure supplement 1. Both germline and conditional deletion of Styxl2 caused lethality.

(A) The schematic of the genomic locus of mouse Styxl2 and the targeting strategy. The probes used for Southern blot are indicated by black bars. (B, C) The schematic of the mating strategy to generate Styxl2 germ-line knockout (KO) mice was shown (B). The number of live pups (B) and that of embryos (C) of different genotypes at different embryonic stages were counted and presented. (D) The schematic of the mating strategy to generate conditional Styxl2 KO mice and the number of live pups (in brackets) older than 1 day were shown. (E) Hindlimb muscle sections from control (Ctrl) and Styxl2 conditional KO (CKO) P1 mice were subjected to electron microscopy analysis. Scale bar: 1 μm.