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Figure Caption
Fig. 1 Flow Chart of FSEC-TS The protein sample, after heat treatment and centrifugation, is loaded onto a SEC column, connected to a fluorescence detector to monitor GFP or tryptophan fluorescence. The panel labeled ‘‘Fluorescence’’ shows a hypothetical elution profile from GFP or tryptophan fluorescence.
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Reprinted from Structure (London, England : 1993), 20, Hattori, M., Hibbs, R.E., Gouaux, E., A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening, 129312991293-9, Copyright (2012) with permission from Elsevier. Full text @ Structure