FIGURE 2

FIGURE 2

LC–MS‐based isotope tracking of yolk sac membrane (YSM) in stage 31. Abundance of M + 3 isotopologues of glucose, glucose‐6‐phosphate (‐P), fructose‐6‐P, glucose‐1‐P, and sedoheptulose‐7‐P in the YSM after 3‐h incubation with ¹³C‐labeled substrates. Horizontal axes show each ¹³C‐labeled substrate and the control without them, and vertical axes show the levels of M + 3 (as % M + 0). Data are presented as mean ± standard error (N = 9), and asterisks (*) indicate significant differences (p < 0.05) between the control and each group. Tests for significant differences were conducted with one‐way ANOVA followed by Dunnett's test after log‐transformation of the values. N/A, not available.