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Fig. 5 il1r1 crispants rescue glut1b:mCherry expression in brain endothelial cells during CNS angiogenesis (A) Representative confocal microscopy images showing rescue of glut1b:mCherry expression in il1r1 crispants. CNS/Il-1?, kdrl:EGFP, glut1b:mCherry embryos were injected with CRISPR/Cas9 RNP complexes (cr1 and cr2) at the one-cell stage. Control embryos and il1r1 crispants were either untreated (No Dox) or treated (10.0 ?g/mL Dox) at 6 hpf and then imaged at 52 hpf (dorsal view; anterior left). Scale bars are 100 ?m. (B and C) Quantification of the number of CtAs (B) and average glut1b:mCherry fluorescence intensity (C) in the hindbrain vasculature of control (CRISPR ?) and il1r1 crispants (CRISPR +) either untreated (Dox ?) or treated with 10.0 ?g/mL Dox (Dox +) (n = 5 for each condition). Error bars in B and C represent means ± SEM (?p < 0.05; ??p < 0.01; ???p < 0.001; no label = not significant).