ZFIN Image: Wang et al., 2023, Fig. 5

Image

Figure Caption

Fig. 5 RCL1 overexpression rescues 18S rRNA production and cell growth after BMS1 knockdown. (A and B) Cell growth of Hela (A) or HepG2 (B) cells as indicated was determined by using Cell Counting Kit-8 every 24 h from 0 to 96 h. For each time point, five repeats of one sample were set. (C) Diagram illustrating human pre-rRNA processing pathway 1. Probes used for northern blot analysis were shown as P1 and P2. (D and E) Northern blot analysis of pre-rRNA processing using biotin-labeled P1 and P2 probes in Hela (D) and HepG2 (E) cells after doxycycline treatment. Methylene blue staining was used to indicate 28S and 18S rRNA levels. (F and G) Detection of 18S/28S rRNA ratio by Agilent 2100 in Hela (F) and HepG2 (G) cells. ***P < 0.001; *P < 0.05.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Mol. Cell Biol.