Figure 3

Figure 3

Effects of PH on oxidative stress and neutrophil activity in the kras+ zebrafish liver during the kras^G12V-induced development of HCC. (A) IHC staining of HIF1α in PH/sham surgery-treated kras+ livers on Day 5. (B) Quantification of HIF1α-positive liver tissue based on (A) (n = 5). The HIFα positive fraction was determined by measuring the DAB-stained area versus the total tissue area with imageJ plugin color deconvolution. (C) Expression of hif1aa in PH/sham surgery-treated kras+ livers on Day 3 as determined by RT-qPCR (n = 5). (D) Expression of hif1aa in WT male livers within 72 h after PH/sham surgery as determined by RT-qPCR (n = 3). (E) Fluorescence images of PH/sham surgery-treated kras/lyz+ livers following Dox induction. (F) Quantification of DsRed+ neutrophils in the liver based on (E) (n ≥ 4). (G) Expression of cybb in PH/sham surgery-treated kras/lyz+ livers on Day 3 as determined by RT-qPCR (n = 4). Scale Bar = 20 μm. Circles, squares, and triangles in (B,F) indicate values of individual samples. ns p > 0.05, * p ≤ 0.05, ** p ≤ 0.01.