Figure 2

Figure 2

Effects of PH on the progress of HCC-associated characteristics in kras+ zebrafish. (A) IF staining of PCNA and HNF4α in the PH/sham surgery-treated kras+ zebrafish liver 3 days after Dox induction. (B) Quantification of PCNA and HNF4α-double positive proliferating hepatocytes based on (A) (n = 4). Circles indicate values of individual samples. (C–E) Expression of (C) ccnb1 and cdk1, (D) col1a1b, lama5, (E) acta2 and tgfb1b in the PH/sham surgery-treated kras+ zebrafish livers 3 days after Dox induction as determined by RT-qPCR (n = 4). (F) Expression of kras^G12V in the PH/sham surgery-treated kras+ zebrafish livers 3 days after Dox induction as determined by RT-qPCR (n = 3). Scale Bar = 20 μm. ns p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.