ZFIN Image: Zhao et al., 2024, Fig. 3

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Figure Caption

Fig. 3

Deficiency of P2RY11 reduces the HCRT expression. A, Reduced expression of hcrt mRNA in P2RY11^−/− mutants at 3 dpf detected by RT-qPCR (n = 20). B, Western blot (n = 10) and statistical analysis (n = 3) of the HCRT protein in P2RY11^−/− mutants at 3 dpf detected by western blot. C, Decreased HCRT neurons in P2RY11 MO injected larvae at 3 dpf (n = 20). D, Reduced expression of hcrt mRNA in P2RY11 MO morphants at 3dpf (n = 20). E, Western blot (n = 20) and statistical analysis (n = 3) of the P2RY11 protein in P2RY11 MO morphants at 3dpf. T-test (A, B, E) and one way ANOVA (D) were conducted. *p < 0.05, **p < 0.01 ***p < 0.001

Acknowledgments

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