Fig. 1

Fig. 1

Phylogenetic trees of the P2YR family and the generation of a P2RY11^−/− mutation in zebrafish using CRISPR-Cas9 editing. A. Phylogenetic analysis was conducted on the predicted protein sequences of 8 families of P2Y receptors across 7 species using the neighbor-joining method. B. Structure of the zebrafish P2RY11 gene and protein. A guide RNA was designed to specifically target exon 2. The P2RY11^−/− allele was created by deleting 8 bases in exon 2. The "-" indicates the deleted nucleotides. The mutation results in a frameshift and premature truncation of the protein. The blue boxes represent the transmembrane domains of P2RY11 protein. C. Western blot and statistical analysis (n = 3) of the P2RY11 protein in the tail of the P2RY11^−/− and P2RY11^+/+ adult (3 mpf) zebrafish. D. The expression level of P2RY11 mRNA in the 3-dpf P2RY11^−/− and P2RY11^+/+ larvae was analyzed by qRT- PCR (n = 20). T-test, ***p < 0.001, **** p < 0.0001