Fig. 6

Fig. 6 Dopamine D2-like receptor signaling modifies visual size tuning of gad1b+ and gad1b− pcp4a+ PVNs (A) Bar graph depicting percentages of pcp4a+/gad1b+ PVNs, imaged in two sessions 3 h apart, classified as persistent, lost, and gained in quinpirole-treated and untreated (control) 7 dpf elavl3:H2B-GCaMP6s larvae. n = 4 fish per group. (B) Graph showing tuning size (WMR angle) of persistent pcp4a+/gad1b+ PVNs before and after quinpirole treatment. n = 24 neurons from 4 larvae. (C) Graph displaying percentages of persistent pcp4a+/gad1b+ PVNs changing response type in the two imaging sessions. Small, neurons responding to circles ≤5°; large, neurons responding to circles ≥10°; dual, neurons responding to both types of stimuli. n = 4 larvae per group. (D) Graph showing percentages of persistent, lost, and gained pcp4a+/gad1b− PVNs in quinpirole-treated and untreated (control) larvae. n = 4 fish per group. (E and F) Bar graphs depicting size tuning (WMR angle) of lost (E) or gained (F) pcp4a+/gad1b− PVNs in fish treated with quinpirole and untreated controls. nLost-Control = 55 neurons from 4 larvae, nLost-Quinpirole = 76 neurons from 4 larvae, nGained-Control = 82 neurons from 4 larvae, nGained-Quinpirole = 34 neurons from 4 larvae. ∗p = 0.03; n.s., not significant; nested two-way ANOVA. (G) Graph showing WMR angles of persistent pcp4a+/gad1b− PVNs before and after quinpirole treatment. n = 77 neurons from 4 larvae. (H) Graph displaying percentages of persistent pcp4a+/gad1b− PVNs changing response type in the two imaging sessions. n = 4 larvae per group. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001; n.s., not significant; t test with Bonferroni-Holm correction (A, C, D, and H), paired t test (B and G), or nested two-way ANOVA (E and F). Data are shown as mean ± standard error of the mean.