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Fig. 6 Washout of theophylline in transgenic DIVE expressing zebrafish line. Representative brightfield and fluorescent micrographs from embryos containing the integrated biosensor plasmid (Ubi:GFP-DIVE_6; Ubi:mCherry) and bathed for 24 hours in 1 mM theophylline. Embryos were measured after removal of theophylline. Zebrafish orientation is lateral view, anterior left. Scale bar: 250 μm. Graph displays quantification of pixel intensity calculated for the trunk of the zebrafish with the GFP/mCherry ratio plotted. The average GFP/mCherry ratio ± s.d. with values for individual fish shown.
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