Figure 4

Figure 4

MnCl₂ exposure reduces Nrgn expression in the brain. (a–c) Representative images of Nrgn immunostaining of brains at 5 dpf (lateral views). (a) Negative control (NC)—unexposed larva. (b) Larva exposed to MnCl₂ (500 µM) from 2.5 hpf to 5 dpf. (c) Larva exposed to MnCl₂ (500 µM) from 2.5 hpf to 2 dpf. ca. anterior commissure. Ce, cerebellum; H, hypothalamus; NC, negative control; MO, medulla oblongata; OB, olfactory bulbs; OT, optic tectum; Po, preoptic area; Tel, telencephalon. Scale bar: 100 µm. (d) Neurogranin mean fluorescence intensity levels (Relative Unit Intensity (RUI)) at 5 dpf following MnCl₂ exposure (500 µM). One-way ANOVA. * p = 0.048; ns, not significant. n = 8 larvae per condition. Data presented as mean ± SD. (e) mRNA expression of nrgna and nrgnb at 4 dpf upon MnCl₂ exposure (500 µM) from 2.5 hpf. Student’s two-tailed t-test. nrgna: ** p = 0.026; nrgnb: *** p = 5.51 × 10⁻⁵. n = 6 per condition. Data presented as mean ± SD.