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Fig. 2 Pcgf5 is involved in the regulation of RA-induced neural induction in P19 cells. (A-C) qPCR and Western Blot was used to analyse the interference efficiency of Pcgf5. (D,E) Representative images show immunofluorescence of Sox2 and Oct4 from Con and Pcgf5-KD after 0.5 μM RA induction. Scale bar, 5 μm. (F,G) Quantification of (D) and (E). More than 100 cells were counted in each experiment. (H,I) Following the knockdown of Pcgf5, qPCR was utilized to evaluate the expression of Zfp521, Pax6 and Oct4, Nanog. (J,K) Western Blot was used to detect the expression of Pax6, Sox2 and Oct4 after Pcgf5 knockdown. Full-length gels before cropping are noted in Fig. S5. Data are presented as means ± SEM of at least three independent experiments (A,C,H–I,K), * (p < 0.05), ** (p < 0.01),*** (p < 0.001), **** (p < 0.0001).