Figure 6.

Figure 6.

Conservation of chromatin state transitions between zebrafish and neonatal mice after heart injury. (A) Schematic representation of the experimental flow to identify converted regulatory regions in zebrafish (danRer11) and mouse (mm10) genomic areas. (B, C) Average H3K27ac ChIP-seq profiles of neonatal hearts at different time points (day D1.5, D3 and D7) after inducing myocardial infarction (MI) at 1 day post birth (P1) at conserved regions showing Ia_A (B) and U_Pa transitions at 4 dpci (C) (7,8) (GSE123868). (D) Genome tracks of H3K27ac ChIP-seq reads of neonatal hearts at different time points after MI at representative genes. (E) Enriched GO terms in genes associated with the U_Pa (cyan) and the Ia_A chromatin state transitions (beige). The P-value was determined by enrichGO from clusterprofiler after the ORA test; P-value *P < 0.05. (F) Heatmap displaying the enriched DNA binding proteins associated with the conserved chromatin state transition groups. (G) Heatmap displaying the expression of transcription factors associated with conserved Ia_A, U_Ea and U_Pa chromatin state transitions, and downstream regulatory genes at D3 after MI at P1 in single-cell RNA sequencing datasets from neonatal hearts (GSE153481). (H) Heatmap displaying the expression of transcriptional factors associated with conserved Ia_A, U_Ea and U_Pa chromatin state transitions, and downstream regulatory genes at D3 after MI in CM single-cell data (GSE130699).