Figure 5.

Figure 5.

RNA-seq analyses uncover significantly down-regulated biological processes and pathways and highlight deficiency of erythroid differentiation common in both mbd5^ΔMBD KO and mbd5 KD embryos. (A) Schematic of staged embryo collection and resulted differentially expressed genes in mbd5^ΔMBD KO and mbd5 KD samples at 52 hpf at the indicated threshold. (B) Venn diagrams of genes downregulated (left) and up-regulated (right) in KO and KD compared to AB WT. (C–E) Gene ontology (GO) analysis of commonly downregulated genes shown in (B, left) reveals significantly altered biological processes (C), cellular components (D) and molecular functions (E) upon disruption of mbd5. (F) Protein-associated networks analysis (https://string-db.org/) of commonly downregulated genes shown in (B, left) highlights a deficit in erythroid differentiation. (G, H) O-Dianisidine staining of hemoglobin at 70 hpf and quantification of relative staining intensity. P= 0.035, Student's t-test. (I) Images of hbae1 in situ show reduced expression in mbd5^ΔMBD KO larvae. Scale bar, 200 μm (G, I).