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Fig. 7 Comparison of the reconstructed videos using our method and a mutual information approach, 4 dpf transgenic Fli1V/Myl7mR zebrafish heartbeat. Cross marks highlight anatomically implausible spots where myocardium (red) intersects with endocardium (green). (a) With high number of frames both methods give similar results, but mutual information gives artifacts with An external file that holds a picture, illustration, etc. Object name is S2633903X23000223_inline233.jpg , while our method is not impacted. (b) When working on small images, mutual information gives incorrect channel synchronization between red and green, while our method generates a plausible solution. See also Supplementary Videos 2–4.