ZFIN Image: Lai et al., 2024, Fig. 2

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Figure Caption

Fig. 2

Knockdown of sdc4 expression decreased the scar deposition of zebrafish hearts. Acid fuchsin orange G (AFOG) staining of heart sections was used to visualize fibrin (red), myocardium (light orange), and collagen (blue). Dashed line: post-cryoinjury area. Data showed scar deposition both in the negative control group and sdc4 siRNA group at 3 dpci (A) and 7 dpci (C). The hearts of the negative control group contained abundant collagen at the injury area at 7 dpci. However, in the sdc4 siRNA group, the scar was nearly completely resolved and replaced with new muscle. The statistical results showed that the scar deposition percentage of zebrafish hearts after cryoinjury did not change at 3 dpci (B) but significantly reduced in the sdc4 siRNA group compared to the negative control group at 7 dpci (D). Scale bar: 200 μm (****p < 0.001 vs. negative control; n = 3–5 per group) (dpci: days post cryoinjury)

Acknowledgments

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