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Fig. 5 Effects of MS33C, MS34C and MS33A treatments on cardiovascular development. (A) At 72 hpf, chemical treatment with MS33C and MS34C up to 200 μM had no effect on normal heart development. However, zebrafish larvae treated with MS33A showed heart edema at 50 μM but not at 25 μM. The heart region is indicated by arrow. Anterior is to the left, lateral view. n = 5 larvae for each concentration. (B) Examination of blood vessel development in living embryos using a transgenic zebrafish line, Tg(kdrl:egfp), at 30 hpf. Growing intersegmental vessels (ISVs, arrows) in the trunk region were labelled by fluorescent GFP expression under control of the endothelial cell-specific kdrl gene promoter. MS33C and MS34C treatment had no effect on normal vessel development up to 200 μM. However, MS33A affected ISVs development at 50 μM but not at 25 μM. n = 3 embryos for each concentration. Scale bar = 200 μm.