Fig. 4 Effects of MS33C, MS34C and MS33A treatments on heart rate of zebrafish larvae at 72 hpf. (A) MS33C-treated larvae exhibited normal heart beats up to a high concentration of 200 μM. (B) MS34C- treated larvae displayed normal heart beats up to a high concentration of 200 μM. (C) MS33A-treated larvae showed a decrease in heart beats from 25 μM to 50 μM compared to control 0.1% DMSO-treated larvae. n = 10 larvae for each concentration of tested compounds. The graphs shown are representative of three independent experiments. Mean values were expressed as bar graphs; ns indicates not significant; ****p < 0.0001.
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