Fig. 5

Fig. 5 The immune response is hampered in cd74a; cd74b mutants.

a Experimental design for bulk RNA-seq analysis of injured ventricles from wild types and cd74a; cd74b mutants at 120 hpci. b Volcano plot showing differentially expressed genes (FC ≥ 2; FDR < 0.05). Blue and red dots represent genes down- and upregulated, respectively, in cd74a; cd74b mutants compared with wild types. Blue and red values represent the number of significantly down- and upregulated genes, respectively; Wald test, corrected for multiple testing using the Benjamini-Hochberg method. c, d Heat maps showing the relative expression of genes encoding known immune-related factors (c), and proteins involved in T cell activation and survival (d) in individual samples. e–g Gene enrichment analyses showing significantly regulated Gene Ontology terms (e), Reactome (f) and KEGG pathways; immune-related processes and pathways marked in red. FDR false discovery rate, FC fold change, WT wild-type samples, Mut cd74a; cd74b mutant samples.