Fig. 2

Fig. 2 One endocardial cell subcluster exhibits an antigen presentation and immune-like transcriptome.

a Experimental design for subclustering analysis of EdCs from a published scRNA-seq dataset of the whole regenerating zebrafish heart⁴³. b UMAP plot showing 12 different EdC subclusters. c Dot plot showing enriched expression of antigen presentation genes in at least subcluster 10. d List of top 40 marker genes in subcluster 10, showing strong enrichment in immune-related genes (red). Wilcoxon Rank Sum Test; adjusted P value based on Bonferroni correction. e Box plot showing the increased relative abundance of cells in subcluster 10 during regeneration compared with uninjured; box plot represents the median, Q1, Q3, the minimum, and the maximum; dots represent potential outliers; n = 5, 10, 13, 5 independent datasets for UI, 72 hpci, and 7 and 14 dpci, respectively (see⁴³); one-way ANOVA (P value included in the graph). f Plot showing the average expression of antigen presentation genes within subcluster 10 at different time points, revealing increased levels during regeneration. UI uninjured.