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Fig. 3 Impact of cyclin F enzymatic activity on TDP-43 depletion and cell toxicity. A. Comparison of cyclin FWT and cyclin FLP/AA ubiquitylation cascade. Cyclin FWT can associate with the SCF complex leading to the ability to mediate ubiquitylation of substrates whilst cyclin FLP/AA has reduced association with the SCF complex and thereby has reduced enzymatic activity. B. Effect of mCherry, mCherry-cyclin FWT and mCherry-cyclin FLP/AA on TDP-43-GFP MFI. C. Expression of mCherry constructs. D. Effects of cyclin F enzymatic activity on the percentage of SYTOX positive cells. A Student's t-test was performed to determine significance. N = 3. *p ≤ 0.05, **p ≤ 0.01., ****p ≤ 0.0001, ns; not significant. All data represent the mean along with SD.