Fig. 6

Fig. 6 R621S and L819V mutations of RB1 may play a role in neuronal apoptosis.

A AO staining in the brain at 3 dpf after injecting with control, hRB1 mRNA, hRB1^R621S mRNA, and hRB1^L819V mRNA in siblings and zrb1-KO mutants. The white dotted line outlines the cerebellum and myelencephalon. A′ Quantification of AO⁺ cells in all groups of (A) (one-way ANOVA; mean ± SEM; ****P < 0.0001; ns, not significant; n ≥ 10). B Co-immunoprecipitation (Co-IP) analyses on the interaction between hRB1/ hRB1^R621/hRB1^L819V and Kmt5b. 293T cells were transfected with GPF–Kmt5b and mCherry-hRB1, mCherry-hRB1^R621 or mCherry hRB1^L819. Immunoprecipitation was performed with an antibody against GPF (upper panel) and confirmed with reciprocal immunoprecipitation experiments with antibodies against mCherry (lower panel). IgG represents a control antibody used for IPs. Input lanes contain lysate equal to one fifth of the amount used for the pull-down assays. IP indicates the antibody used for immunoprecipitation.