|
Fig. 7 Influence of nat10 KD on color preference and retinal morphology in zebrafish. (A) Representative heat map images showing the innate color preference behavior of zebrafish in a 10 minutes test within a plus-maze, each arm of which is covered with red, blue, yellow, and green sleeves; upper and lower panels for the NC and the nat10 KD zebrafish larvae, respectively. (B) The calculation of the color choice index is based on the ratio of time spent in each two-color combination. Results are presented as mean ± SEM from 10 independent experiments. Statistical analysis was performed using 2-way ANOVA with the Tukey post hoc test; **P < 0.001, ***P < 0.001 vs, NC. (C) Coronal sections of the retinas from the NC (upper panel) and the nat10 KD (lower panel) zebrafish larvae; GCL, ganglion cell layer; INL and ONL, inner and outer nuclear layer, respectively; IPL and OPL, inner and outer plexiform layers. (D) Immunofluorescence staining of the retinas of the NC (left panel) and the nat10 KD (right panel) zebrafish larvae with elavl3 (red) and DAPI (blue).