ZFIN Image: Ugolini et al., 2024, Fig. 5

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Figure Caption

Fig. 5 Loss of mir430 transcription bodies causes ectopic transcription bodies to go into elongation.

a, Visualization of RNAPII-Ser2P and RNAPII-Ser5P with Fabs in WT and mir430^−/− + inj MiR430 embryos at 512- and 1,024-cell stages. Shown are representative micrographs of individual nuclei. See Extended Data Fig. 6 for complete cell cycles. N = 3 biologically independent experiments. b, Quantification of Ser5P-positive/Ser2P-negative (red), Ser5P-positive/Ser2P-positive (yellow) and Ser5P-negative/Ser2P-positive (green) transcription bodies during the cell cycle at 512-cell and 1,024-cell stages as shown in a. Number (upper panel) and percentage (lower panel) are shown. N = 3 biologically independent experiments, number of nuclei (n) analysed at each timepoint, in each cell cycle and in each condition (WT and mir430^−/− + inj MiR430) are reported. Source numerical data are available in Source data.

Source data

Acknowledgments

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