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Fig. 3 Detection of single nucleotide differences at the slc45a2 locus by GIMA. (a) Genotyping of the slc45a2 locus using GIMA. H, heterozygous alleles; M, homozygous mutant alleles; W, homozygous wild-type alleles. The black arrowhead indicates the position of the PCR amplicons (heterozygous, homozygous mutant, and homozygous wild-type bands). The red arrowheads indicate the position of the GIP?mutant or the GIP (30 bp)?mutant complex. The green arrowheads indicate the position of the GIP?wild-type or the GIP (30 bp)?wild-type complex. The red asterisk and green asterisk indicate the positions of the AIP?mutant and AIP?wild-type complexes, respectively. The molecular marker is indicated on the right. Essentially similar results were obtained from at least three independent experiments. (b) Nucleotide sequences of the wild-type slc45a2 sense strand, the mutant slc45a2 sense strand, GIP, and AIP are shown. Green, wild-type nucleotide site; red, mutant nucleotide site; blue, inserted guanine nucleotides; pink, inserted adenine nucleotides.